normal phase chromatography process
IRI: https://spec.industrialontologies.org/ontology/construct/NormalPhaseChromatographyProcess
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/
SubClass Of: liquid chromatography process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › chromatography process › liquid chromatography process › normal phase chromatography process
Definition
liquid chromatography process in which the stationary phase is polar and the mobile phase is nonpolar, resulting in the separation of components based on their relative polarity
Explanatory Notes
This process is commonly used to separate small, polar organic compounds. Components with higher polarity interact more strongly with the stationary phase and elute later, while less polar components elute earlier with the nonpolar mobile phase.
Examples
- A phospholipid extract was separated into distinct lipid classes using a polar silica stationary phase and a mobile phase consisting of hexane and isopropanol; A racemic small molecule API was purified to isolate the active enantiomer using a chiral polar stationary phase and a mobile phase of heptane and ethanol; A glycan mixture derived from enzymatic deglycosylation was separated based on polarity using an amino-functionalized silica stationary phase and an acetonitrile–water mobile phase
Adapted From
- AFO_/process#AFP:0002328
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
Insufficient constructs are present to create a set of necessary and sufficient conditions
Formal Axioms
First-Order Logic Axioms
NormalPhaseChromatographyProcess(x) → LiquidChromatographyProcess(x)
Semi-Formal Natural Language Axioms
if x is a ‘normal phase chromatography process’ then x is a ‘liquid chromatography process’