cultured cell
IRI: https://spec.industrialontologies.org/ontology/construct/CulturedCell
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/
SubClass Of: cell
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › bfo:object › cell › cultured cell
Definition
cell that is grown, maintained, or adapted to grow in a controlled artificial environment that supports its survival and proliferation
Explanatory Notes
1)Cultured cells are typically immortalized, meaning they retain the capacity to proliferate indefinitely under suitable growth conditions; in contrast, primary cells such as those directly isolated from a patient without genetic or oncogenic modification undergo a limited number of mitotic divisions before entering senescence or undergoing cell death 2) A blurred line would be the case of the CAR-T cell therapy situation where the cells are often taken directly from the patient, modified (not immortalized), expanded in a bioreactor and then infused back into the patient. In this case, CAR-T cells are still considered cultured cells because they are subjected to ex vivo cultivation under controlled, artificial conditions. After being isolated from a patient (typically as primary T cells), they are genetically modified and then expanded in bioreactors or culture vessels using defined media, gas control, and temperature regulation. Although they are not immortalized, they undergo proliferation outside their native environment. This fulfills the defining criteria of a cultured cell as one that grows in an artificial, controlled setting. Their classification as cultured reflects the environmental and procedural context, not the permanence or origin of the cell line.
Examples
- CHO-K1 cell;HeLa cell
Counter-Examples
- Freshly isolated hepatocyte; Hematopoietic stem cell isolated from bone marrow
Adapted From
- https://www.thermofisher.com/rs/en/home/references/gibco-cell-culture-basics/introduction-to-cell-culture.html and http://purl.obolibrary.org/obo/CL_0000010
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are currently inssuficient constructs to adequately represent adapt to grow in artificial environment.
Formal Axioms
First-Order Logic Axioms
CulturedCell(x) → Cell(x)
Semi-Formal Natural Language Axioms
if x is a ‘cultured cell’ then x is a ‘cell’
Description Logic
SubClassOf: constr:Cell