mixed mode chromatography medium
IRI: https://spec.industrialontologies.org/ontology/construct/MixedModeChromatographyMedium
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaEquipment/
SubClass Of: chromatography medium
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › chromatography medium › mixed mode chromatography medium
Definition
chromatography medium with immobilized functional groups designed to selectively interact with molecules through multiple distinct interaction mechanisms
Explanatory Notes
Different modes of interaction can occur between the chromatographic ligand and the target molecule depending on the experimental conditions. These interactions may function cooperatively or independently. For example, in the case of mixed-mode pH-controllable sorbents, the ligand 4-mercaptoethylpyridine (MEP) exhibits condition-dependent binding behavior. At neutral and basic pH, the pyridine ring remains uncharged, enabling the ligand to act as a hydrophobic binding moiety. As the pH decreases, the nitrogen in the pyridine ring becomes protonated, introducing a positive charge and thereby enabling ionic interactions. This transformation renders the resin a mixed-mode medium. The dual functionality of MEP under different pH conditions exemplifies a chromatographic mode known as hydrophobic charge induction chromatography (HCIC), where hydrophobic binding at neutral pH transitions to charge-mediated elution at lower pH.
Examples
- Hydroxyapatite (CHT/CFT/Bio-Gel HT/HTP) Resins that interact through electrostatic and calcium coordination complexes; Resins with hydrophobic ion exchange ligands
Synonyms
- multimodal chromatography medium
Adapted From
- http://wolfson.huji.ac.il/purification/PDF/HCIC/GE_MultimodalChromat.pdf and https://www.bio-rad.com/en-rs/applications-technologies/introduction-multimodal-or-mixed-mode-chromatography?ID=LUSN9AKG4
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs in the ontology to create necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
MixedModeChromatographyMedium(x) → ChromatographyMedium(x)
Semi-Formal Natural Language Axioms
if x is a ‘mixed mode chromatography medium’ then x is a ‘chromatography medium’