capture step
IRI: https://spec.industrialontologies.org/ontology/construct/CaptureStep
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/
SubClass Of: purification process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › manufacturing process › purification process › capture step
Definition
purification process in which target molecules are selectively and reversibly retained while impurities are allowed to pass through in the flowthrough or wash fractions
Explanatory Notes
The reversible nature of retention implies that conditions such as buffer composition or pH can be adjusted to reverse the binding of the target molecule, allowing its release from the capture medium and enabling purification to continue.
Examples
- A capture purification process uses protein A chromatography to retain monoclonal antibodies while host cell proteins flow through; A capture purification process applies ion exchange chromatography to bind recombinant proteins from cell culture supernatant; A capture purification process employs affinity membranes to isolate His-tagged proteins from bacterial lysates; A capture purification process uses mixed-mode chromatography to retain viral vectors while impurities are washed away;
Counter-Examples
- A flowthrough chromatography process removes DNA and endotoxins while the target protein does not bind to the column; A depth filtration process removes cells and debris without selectively retaining the target molecule;
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
Insufficient constructs are present to create a set of necessary and sufficient conditions
Formal Axioms
First-Order Logic Axioms
CaptureStep(x) → PurificationProcess(x)
Semi-Formal Natural Language Axioms
if x is a ‘capture step’ then x is a ‘purification process’