chromatography medium

IRI: https://spec.industrialontologies.org/ontology/construct/ChromatographyMedium

Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaEquipment/

SubClass Of: bfo:material entity

Class Hierarchy

owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › chromatography medium

Direct subclasses:

• affinity chromatography medium
• anion exchange chromatography medium
• cation exchange chromatography medium
• hydrophobic chromatography medium
• mixed mode chromatography medium
• size exclusion chromatography medium

Definition

material entity designed to selectively and reversibly retain different molecules during one or more chromatography processes

Explanatory Notes

1) This material is typically engineered to enable separation based on physicochemical properties such as charge, size, hydrophobicity, or specific affinity. It functions by interacting with analytes in a mobile phase as they pass through or across the matrix. The material may be functionalized with ligands or surface groups to enhance selectivity and is commonly packed into columns or cartridges used in chromatographic systems. 2) Chromatography media can take various physical forms including resins, monoliths, and membranes. Resins typically consist of spherical or irregularly shaped porous beads that serve as the stationary phase. Monoliths are continuous, single-piece porous structures that allow high flow rates and low backpressure. Membranes are thin, porous films that enable selective retention based on size or chemical affinity, often used for rapid separations. All serve as porous matrices designed to selectively and reversibly retain target molecules during chromatography processes. 3) This definition reflects the canonical design of chromatography media, which are typically engineered to support selective and reversible interactions with molecular species, enabling separation, recovery, or analysis. In certain use cases, such as affinity depletion workflows, the chromatography medium may be operated in a process mode where bound targets are not eluted and are instead discarded with the medium. In such cases, the binding appears irreversible not because of a limitation in the medium’s capability, but because the process is intentionally run without an elution step. The underlying binding interactions remain chemically reversible and could be reversed under appropriate conditions.

Examples

• affinity chromatography medium; size exclusion chromatography medium; anion exchange medium

Primitive Class

This class is declared primitive and it does not have necessary and sufficient condiftions defined.

Primitive Rationale

There are insufficient constructs in the ontology to create necessary and sufficient conditions.

Formal Axioms

First-Order Logic Axioms

ChromatographyMedium(x) → MaterialEntity(x)

Semi-Formal Natural Language Axioms

if x is a ‘chromatography medium’ then x is a ‘material entity’

Description Logic

constr:ChromatographyMedium ⊑ bfo:material_entity

constr:ChromatographyMedium ⊓ constr:ChromatographyColumn ⊑ ⊥

SubClassOf: bfo:material_entity

constr:ChromatographyMedium DisjointWith: constr:ChromatographyColumn