affinity chromatography medium
IRI: https://spec.industrialontologies.org/ontology/construct/AffinityChromatographyMedium
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaEquipment/
SubClass Of: chromatography medium
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › chromatography medium › affinity chromatography medium
Direct subclasses:
Definition
chromatography medium which has an immobilized ligand as its part which has reversible binding capability to a specific part of a biomolecule
Explanatory Notes
In chromatography, functional groups refer broadly to chemical moieties attached to the stationary phase that provide general interaction types with solutes, such as ionic or hydrophobic interactions. Ligands are a specialized subset of functional groups, typically larger and more complex molecules covalently attached to the medium to enable highly selective and specific binding to target molecules through molecular recognition. In affinity chromatography, ligands are used rather than general functional groups to emphasize this specificity, reflecting the medium’s design for selective capture of particular biomolecules.
Examples
- Protein A Resin typically used for IgG capture; Con A Sepharose resin used for Mannosylated and glucosylated glycoproteins; IMAC resins for capturing proteins with a his-tag as their part; Sterptavidin agarose used for capturing biotinilyated proteins or nucleic acids
Adapted From
- http://wolfson.huji.ac.il/purification/PDF/affinity/GE_Affinity_Chromatography_Handbook-Antibodies.pdf
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs in the ontology to create necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
AffinityChromatographyMedium(x) → ChromatographyMedium(x)
Semi-Formal Natural Language Axioms
if x is an ‘affinity chromatography medium’ then x is a ‘chromatography medium’