mobile phase

IRI: https://spec.industrialontologies.org/ontology/construct/MobilePhase

Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaEquipment/

SubClass Of: bfo:material entity

Class Hierarchy

owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › mobile phase

Definition

material entity with a mobile phase role

Semi-Formal Definition:

every instance of ‘mobile phase’ is defined as exactly an instance of ‘material entity’ that ‘has role’ some ‘mobile phase role’

Examples

  • Water as mobile phase in reverse-phase chromatography; acetonitrile used as mobile phase in HPLC; phosphate buffer as mobile phase in ion-exchange chromatography; methanol serving as mobile phase in normal-phase chromatography; gradient of water and acetonitrile as mobile phase in gradient elution; sodium chloride solution as mobile phase in size-exclusion chromatography

Counter-Examples

  • Liquid used for column cleaning or sanitization after chromatography is completed; Air or nitrogen gas used for drying the column after elution (not used as percolating fluid during chromatography)

Adapted From

  • AFO_/role#AFRL:0000011 and GE Protein Purification Handbook and https://goldbook.iupac.org/terms/view/M03952 and htps://www.sartorius.com/en/knowledge/resources/chromatography-glossary

Formal Axioms

First-Order Logic Definition 

MobilePhase(x) ↔ MaterialEntity(x) ∧ ∃r(MobilePhaseRole(r) ∧ hasRole(x, r))

Description Logic

constr:MobilePhase ≡ bfo:material_entity ⊓ ∃ constr:hasRole .constr:MobilePhaseRole

constr:MobilePhase ⊑ bfo:material_entity

EquivalentTo: bfo:material_entity and constr:hasRole some constr:MobilePhaseRole

SubClassOf: bfo:material_entity